M Lew D. P Wabers H Cooper S. L Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arteriovenous shunts.
Gross M Cramton S. Hufnagel M Koch S Creti R Baldassarri L Huebner J A putative sugar-binding transcriptional regulator in a novel gene locus in Enterococcus faecalis contributes to production of biofilm and prolonged bacteremia in mice.
R Lasa I The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Kjaergaard K Schembri M. Lunsford R. D London J Natural genetic transformation in Streptococcus gordonii : comX imparts spontaneous competence on strain wicky. A Gibbs K. A Hager P. W Phibbs P. Kolter R The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa. T Vidal O Lejeune P Dorel C Developmental pathway for biofilm formation in curli-producing Escherichia coli strains: role of flagella, curli and colanic acid.
Gilmore K. S Srinivas P Akins D. R Hatter K. L Gilmore M. S Growth, development, and gene expression in a persistent Streptococcus gordonii biofilm. H Tang N Aspiras M. B Lau P. C Lee J. P Cvitkovitch D. G A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation.
C Beighton D Hamilton I. R Protein expression by planktonic and biofilm cells of Streptococcus mutans. Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide.
Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. What drives bacteria to produce a biofilm? Oxford Academic. Revision received:. Select Format Select format. Permissions Icon Permissions. Abstract Nearly 40 years ago, Dr. Biofilm , Genetics , Microbial communities. Quorum sensing S.
Alternate sigma factor-stress response S. Regulator involved in slow growth E. Open in new tab. Open in new tab Download slide. Google Scholar Crossref. Search ADS. Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa. Google Scholar PubMed. Regulation of slime production in Staphylococcus epidermidis by iron limitation.
Enterococcus spp. Multiple Streptococcus mutans genes are involved in biofilm formation. Biofilm formation by Staphylococcus epidermidis depends on functional RsbU, an activator of the sigB operon: differential activation mechanisms due to ethanol and salt stress.
Alternative transcription factor sigma B is involved in regulation of biofilm expression in a Staphylococcus aureus mucosal isolate. Identification of the sigB operon in Staphylococcus epidermidis : construction and characterization of a sigB deletion mutant. SarA and not sigma B is essential for biofilm development by Staphylococcus aureus. A mutation in rpoS enhances biofilm formation in Escherichia coli during exponential phase of growth.
Contamination of abiotic surfaces: what a colonizing bacterium sees and how to blur it. Slime production and expression of the slime-associated antigen by staphylococcal clinical isolates. The teicoplanin-associated locus regulator TcaR and the intercellular adhesin locus regulator IcaR are transcriptional inhibitors of the ica locus in Staphylococcus aureus.
Death's toolbox: examining the molecular components of bacterial programmed cell death. Mutation of luxS affects biofilm formation in Streptococcus mutans. Functional genomics approach to identifying genes required for biofilm development by Streptococcus mutans.
The involvement of cell-to-cell signals in the development of a bacterial biofilm. Quorum-sensing control of biofilm factors in Staphylococcus epidermidis. Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia.
Toxins-antitoxins: plasmid maintenance, programmed cell death, and cell cycle arrest. Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes. Are the molecular strategies that control apoptosis conserved in bacteria.
Regulation of the alginate biosynthesis gene algC in Pseudomonas aeruginosa during biofilm development in continuous culture. Biofilm formation on intraocular lenses by a clinical strain encoding the ica locus: a scanning electron microscopy study.
Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. Isolation of an Escherichia coli K mutant strain able to form biofilms on inert surfaces: involvement of a new ompR allele that increases curli expression. Initiation of biofilm formation in Pseudomonas fluorescens WCS proceeds via multiple, convergent signalling pathways: a genetic analysis. Effect of an orphan response regulator on Streptococcus mutans sucrose-dependent adherence and cariogenesis.
Alpha-toxin is required for biofilm formation by Staphylococcus aureus. Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arteriovenous shunts. Key role of teichoic acid net charge in Staphylococcus aureus colonization of artificial surfaces. A kilodalton extracellular protein is essential for the accumulation of Staphylococcus epidermidis strains on surfaces.
A putative sugar-binding transcriptional regulator in a novel gene locus in Enterococcus faecalis contributes to production of biofilm and prolonged bacteremia in mice. The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation.
Natural genetic transformation in Streptococcus gordonii : comX imparts spontaneous competence on strain wicky. The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa.
Establishment of an arbitrary PCR for rapid identification of Tn insertion sites in Staphylococcus epidermidis : characterization of biofilm-negative and nonmucoid mutants. Developmental pathway for biofilm formation in curli-producing Escherichia coli strains: role of flagella, curli and colanic acid. Transcription of clumping factor A in attached and unattached Staphylococcus aureus in vitro and during device-related infection.
Growth, development, and gene expression in a persistent Streptococcus gordonii biofilm. A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. Protein expression by planktonic and biofilm cells of Streptococcus mutans.
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Detecting the presence of bacterial DNA by PCR can be useful in diagnosing culture-negative cases of infection, especially in patients with suspected infection and antibiotic therapy. Ammonia produced by bacterial colonies promotes growth of ampicillin-sensitive Serratia sp. The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.
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Bacterial biofilms are generally defined as structured clusters of bacterial cells enclosed in a self-produced polymer matrix that are attached to a surface.
Bacteria can adhere to cells and tissues as well as to solid surfaces e. Biofilms appear to facilitate the survival of bacterial pathogens in their environment and their host.
Bacterial biofilm isolated from a wide range of niches share several common characteristics: 1 bacterial cells are enclosed in polymer matrix composed of exopolysaccharides, proteins and nucleic acid; 2 biofilm formation is initiated by extracellular signals present in the environment or produced by the bacteria; 3 the biofilm protects bacteria against the host immune response, desiccation and biocides e.
The latter characteristic is of course of great importance in human and veterinary medicine. Despite extensive scientific coverage of biofilms associated with human infections or industrial processes, biofilm formation by bacteria of veterinary importance and associated with zoonotic diseases have received very little attention. My laboratory is studying biofilm formation by Actinobacillus pleuropneumoniae , an important respiratory tract pathogen of swine, and by coagulase-negative staphylococci isolated from bovine mastitis.
We have developed or adapted in vitro models to study biofilms under static conditions or in the presence of a liquid flow. We use screening of library mutants and determination of genes expression to know more on the genes that are important for biofilm formation and dispersion. We also rely greatly on confocal microscopy to visualize and characterize the biofilms.
When infecting the CF lung, P. This is due to the overproduction of the matrix polysaccharide alginate, leading to the formation of a mucoid biofilm that tolerates antibiotics, components of both the innate and adaptive immune response, and resists phagocytosis. The persistence of these mucoid biofilms within the CF lung leads to the development of a distinct antibody response. This prompts chronic inflammation mediated by granulocytes, and results in severe damage to the lung tissue of CF patients see Figure 3 A.
A second example for biofilms in human health is dental plaque potentially leading to dental caries. The consumption of fermentable carbohydrates such as sugary treats or drinks causes an increase in the production and secretion of organic acids by the bacteria found in dental plaque. If left untreated, the increased acidification of the biofilm leads to the demineralisation of the enamel and the formation of dental caries see Figure 3 B.
Due to the widespread distribution of biofilms in diseases and their resilience to numerous antimicrobial treatments, biofilm research is receiving more attention.
Examples include triggering the dispersal of the biofilm or looking into ways of preventing the initial formation, for instance by re-engineering the surfaces they are prone to develop upon such as urinary catheters and implants. Register Log in. Biofilms and their role in pathogenesis Download Biofilms and their role. What is a biofilm? Figure 1 : Polymicrobial biofilm formed by P.
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Rao TS Comparative effect of temperature on biofilm formation in natural and modified marine environment. Aquat Ecol — J Bacteriol 10 — Res Microbiol — Rochex A, Lebeault JM Effects of nutrients on biofilm formation and detachment of a Pseudomonas putida strain isolated from a paper machine. Sauer K, Camper AK Characterization of phenotypic changes in Pseudomonas putida in response to surface associated growth. J Bacteriol — Food Sci Technol — Smith AW Biofilms and antibiotic therapy: Is there a role for combating bacterial resistance by the use of novel drug delivery system?
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